ABSTRACT
Objective To establish a high performance liquid chromatographic ( HPLC) method for determination of gallic acid and ellagic acid in Melastoma dodecandrum. Methods HPLC was applied, the chromatographic column was Waters Xbidge C18(4.6 mm×250 mm,5μm);with methanol-mixed aqueous solution containing 0.3% phosphoric acid and 0.06%tetrahydrofuran as the mobile phrase;gradient elution:0–12 min,1% A ( the detection wavelength was 216 nm);12–24 min, 1% A→55% A (the detection wavelength was 254 nm);flow rate was 1.0 mL.min-1, the column temperature was 30 ℃. Results Good linear relationship of gallic acid and ellagic acid was obtained at ranges of 0.089 7-0.448 μg ( r=0.999 8) and 0.028 4-0.142 μg (r=0.999 8),respectively. The average recoveries were 96.51% (RSD=1.10%) and 96.19% (RSD=1.20%) . Conclusion The method is simple,accurate and repeatable,and it is suitable for content determination of gallic acid and ellagic acid in Melastoma dodecandrum. It provides a basis for quality evaluation of Melastoma dodecandrum.
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Objective: To discuss the quality control method and index of traditional She medicine -Melastoma dodecandrum. Methods:The contents of gallic acid , ellagic acid and total flavonoids ( the total amount of quercetin and kaempferol) were deter-mined by HPLC. Water content, acid insoluble ash content, extract, heavy metals and harmful elements in 26 batches of herbs were also detected. Results:The content range of gallic acid, ellagic acid and total flavonoids was 9. 84-26. 31 mg·g-1 , 4. 13-16. 11 mg· g-1 and 1. 31-38. 28 mg · g-1 , respectively. The content range of water, acid insoluble ash and extract was 6. 70%-13. 90%, 1. 05%-6. 20% and 18. 00%-40. 80%, respectively. The content of Pb in all the samples was more than 5 ppm, the number of sam-ples with the content of Cd above ten thousand three was 7, and the content of As, Hg and Cu in all the samples was less than 1 ppm, ten thousand one and 20 ppm, respectively. Conclusion:The contents of gallic acid, ellagic acid and total flavonoids can comprehen-sively reflect the content of active ingredients in Melastoma dodecandrum. The contents of acid insoluble ash, Pb and Cd in Melastoma dodecandrum are relative high, which should be studied further to draw up reasonable limit.
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Objective:To study the quality standard for Herba Desmodii,and provide the scientific basis for a reasonable method of quality control. Methods:With the methods of microscopic identification,thin-layer chromatography and high performance liquid chromatography for the qualitative and quantitative determination of Herba Desmodii,the ethanol soluble extracts,moisture content and total ashes of Herba Desmodii were determined. Results:The characteristics of thin-layer chromatography were obvious;a good lineari-ty of quercetin and kaempferol was within the range of 0. 002-0. 050 μg(r=1. 000 0)and 0. 004-0. 100 μg(r=0. 999 9)with the av-erage recovery of 97.89%(RSD=0.89%,n=6)and 98.93%(RSD=1.31%,n=6),respectively. Conclusion:The method is simple,accurate and reproducible,which can effectively control the quality of Herba Desmodii.
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In order to reduce heavy burden of cancer on patients, families and social, it needs to raise special funds, apply regular screening for 45~65 years old group, achieve early detection and treatment of cancer, reduce medical expenses and economic loss of cancer greatly, reduce mental burden, establish the cancer prevention input-output model, calculates the economic benefits up to 1:9.89. It is proposed to establish the organization, improve the mechanism, expand the overall costs, secure the screening and popularize early detection and treatment.
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In order to understand the cost of cancer in rural area, to evaluate the cost burden, the project content and treatment status. Typical sampling and random sampling survey are combined to investigate and account major expenditure and economic loss of cancer treatment and related items, analyzes its rationality and scientific, provide references for improving the level of cancer prevention and treatment.